Fig. 3: T-cell receptor (TCR) repertoire analysis in healthy tissue and in tumor microenvironments before and during/after immune checkpoint blockade.

A Simpson’s Clonality (SC) in paired biopsies of patients with treatment naive hepatocellular carcinoma (HCC, (n = 14) and under immune checkpoint blockade (ICB, n = 14), and in biopsies of treatment-naïve Hodgkin Lymphoma (HL, (n = 90) and HL under ICB (subset of HL as paired biopsies under ICB, n = 4). B Clonal expansion of singletons (ES) between two biopsies of reactive lymph nodes (RLN, n = 8), and of HCC and HL pairs as shown in (A). C Clonal expansion of non-singletons (ENS) between two biopsies of RLN (n = 8), and of HCC and HL pairs as shown in (A). D Overlap of TCR sequences as percentage of TCR sequences with the same amino acid sequence in the treatment naive biopsy which were also detected in a follow-up biopsy of the same patient. Box and whiskers plots with median indicated as horizontal bar. ns not significant (p > 0.05), *p 0.05–0.01, **p < 0.01, ***p < 0.0001, ****p < 0.00001.