Fig. 1: Loss of Sting impairs the increased self-renewal of HSPCs mediated by Dnmt3a deficiency. | Leukemia

Fig. 1: Loss of Sting impairs the increased self-renewal of HSPCs mediated by Dnmt3a deficiency.

From: STING mediates increased self-renewal and lineage skewing in DNMT3A-mutated hematopoietic stem/progenitor cells

Fig. 1

A Schematic of the strategy used to generate Dnmt3a; Sting-DKO mice. The phenotypes of these mice were analyzed 16 weeks after poly(I:C) injection. B Deletion of Sting reduced the repopulating capacity of Dnmt3a-deficient LSK cells in CFU assays. LSK cells from different mice were sorted by FACS, and the number of CFUs was counted 7 days after plating. IFNβ was used at a concentration of 1 U/ml. C Loss of Sting suppressed the increased self-renewal of Dnmt3a-deficient LSK cells in vivo (n = 5). D The proportions of myeloid and lymphoid cells in peripheral blood were measured by FACS (n = 5). E Deletion of Sting specifically reduced the proliferation of Dnmt3a-KO HSPCs. The HSPCs were stained with an antibody against Ki67 and analyzed by FACS (n = 6). Data are presented as mean ± s.e.m., with *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001, and “ns” not significant.

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