Fig. 2: Deletion of Sting inhibits the development of CH associated with Dnmt3a deficiency in transplantation models. | Leukemia

Fig. 2: Deletion of Sting inhibits the development of CH associated with Dnmt3a deficiency in transplantation models.

From: STING mediates increased self-renewal and lineage skewing in DNMT3A-mutated hematopoietic stem/progenitor cells

Fig. 2

A Diagram of the whole bone marrow transplantation assay. B Loss of Sting alleviated the splenomegaly in recipients of Dnmt3a-deficient donor bone marrow. The weight of spleens in recipient mice was measured 20 weeks after transplantation. Quantitative data are shown on the right (n = 8). C The plot graph shows the frequency of myeloid cells in the peripheral blood of recipient mice over the course of the transplantation assay (n = 11). D Deletion of Sting inhibited the myeloid differentiation bias mediated by Dnmt3a deficiency. The proportions of myeloid and lymphoid cells in peripheral blood were measured by FACS 20 weeks post-transplantation (n = 8). E Deletion of Sting restrained the self-renewal ability of Dnmt3a-deficient HSPCs. The proportions of LSK cells, LT-HSCs, ST-HSCs, CMPs, GMPs, and MEPs were measured by FACS. Gating plots are shown on the left, and quantitative data are shown on the right (n = 8). Data are presented as mean ± s.e.m., with *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001, and “ns” not significant.

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