Fig. 3: Comparison of growth, migration, and stemness protein expression in H9 CD38^WT and CD38^KO CTCL cell lines.

A Schematic demonstrating development process of CD38^KO H9 CTCL cell line using CRISPR-Cas9, including flow cytometry showing post-sorting purity and CD38- status of CD38^KO line (figure made with Biorender). B CD38^KO CTCL cells showed no growth differences compared to CD38^WT (p = 0.22 by linear regression). C Migration assay comparing CD38^WT and CD38^KO CTCL cells with and without CXCL12 chemoattractant (p < 0.01 by unpaired t test). D Western blot of β-catenin, Bcl6, and Tcf7 protein levels with β-actin loading control comparing between H9 CD38^WT and H9 CD38^KO luciferase CTCL cell lines and healthy donor CD4 + T-cells (HD CD4) imaged via chemiluminescence with a FluorChem Imager and images processed with AlphaView software.