Fig. 1: Phenocycler 2.0TM multiplex imaging schematic showing the sequential steps of oligo-barcode addition, imaging, and washing out the oligo-barcodes. | Leukemia

Fig. 1: Phenocycler 2.0TM multiplex imaging schematic showing the sequential steps of oligo-barcode addition, imaging, and washing out the oligo-barcodes.

From: Multiplex imaging of murine bone marrow using Phenocycler 2.0™

Fig. 1

The process follows these steps: A Addition of oligo-barcode conjugated antibodies on tissue section on a glass slide. B Complimentary oligo-barcode fluorophores bind to their respective barcode conjugated antibodies. C Imaging. D Wash steps to remove oligo-barcode fluorophores. E Sequential application of next set of oligo-barcode fluorophores. F Imaging.

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