Table 2 Antibodies against the cell markers, channels, and oligo-barcode numbers (manufacturer-assigned).
From: Multiplex imaging of murine bone marrow using Phenocycler 2.0™
Antibody | Channel | Conjugated (custom or purchased) | Dilution factor used for staining | Oligo-barcode | Manufacturer Pre-conjugated antibody / Oligo-barcode catalog number |
|---|---|---|---|---|---|
CD31 | 550 | Commercial | 1–100 | 2 | 4250001 |
CD41 | 647 | Custom | 1–200 | 15 | 5550008 |
CD45 | 750 | Commercial | 1–100 | 7 | 4450002 |
CD61 | 647 | Custom | 1–100 | 42 | 5550015 |
CD71 | 647 | Commercial | 1–100 | 27 | 4550111 |
CD117 | 750 | Custom | 1–100 | 19 | 5450002 |
B220 | 750 | Commercial | 1–100 | 10 | 4450006 |
F4/80 | 647 | Commercial | 1–200 | 6 | Leinco-F401 |
Gr1 | 647 | Custom | 1–400 | 3 | 5550017 |
Sca1 | 647 | Custom | 1–200 | 33 | 5550013 |
Ter119 | 550 | Custom | 1–100 | 5 | 5450024 |
CD48 | 750 | Custom | 1–100 | 22 | 5450003 |
CD110 | 750 | Custom | 1–100 | 468 | ** |
CD115 | 550 | Custom | 1–100 | 500 | ** |
Endomucin | 550 | Custom | 1–100 | 41 | 5250008 |
Fibromodulin | 550 | Custom | 1–100 | 40 | 5250017 |
Fibulin2 | 647 | Custom | 1–100 | 31 | 5550004 |
CGRP | 550 | Custom | 1–100 | 518 | ** |
EFEMP2 | 550 | Custom | 1–100 | 35 | 5250007 |
Lumican | 750 | Custom | 1–100 | 34 | 5450007 |
CRTAP | 647 | Custom | 1–100 | 43 | 5550005 |
Col1a | 550 | Custom | 1–100 | 135 | ** |
α-SMA | 647 | Custom | 1–200 | 45 | 5550016 |
Emillin2 | 647 | Custom | 1–100 | 24 | 5550010 |
PEDF | 647 | Custom | 1–100 | 36 | 5550014 |
