Table 3 Preparation of reduction master mix for antibody conjugation.
From: Multiplex imaging of murine bone marrow using Phenocycler 2.0™
No. of antibodies | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|---|---|---|---|---|---|---|---|---|
Reduction solution 1 [µL] | 6.6 | 13.2 | 19.8 | 26.4 | 33 | 39.6 | 46.2 | 52.8 |
Reduction solution 2 [µL] | 275 | 550 | 825 | 1100 | 1375 | 1650 | 1925 | 2200 |
Total [µL] | 281.6 | 563.2 | 844.8 | 1126.4 | 1378 | 1689.6 | 1971.2 | 2252.8 |
