Fig. 4: BCL6-CD74 rearrangements in cases 3 and 4. | Leukemia

Fig. 4: BCL6-CD74 rearrangements in cases 3 and 4.

From: Genetic lesions in nodular lymphocyte-predominant Hodgkin lymphoma and T cell/histiocyte-rich large B-cell lymphoma identified by whole genome sequencing

Fig. 4

A, B Overview of the observed breakpoints in case 3 and case 4 using IGV [68] plots. C Breakpoint-spanning Sanger sequence obtained from the rearrangement in case 3. D Breakpoint-spanning Sanger sequence obtained from the rearrangement in case 4. E Immunofluorescence double staining of BCL6 (red) and CD74 (green) with DAPI counterstaining showing strong nuclear expression of BCL6 in the LP cells of case 3 and weak membrane bound expression of CD74 (arrows). F Immunofluorescence double staining of BCL6 (red) and CD74 (green) with DAPI counterstaining showing strong nuclear expression of BCL6 in the LP cells of case 4 with absence of CD74 expression (arrows). Preserved membrane bound expression of CD74 is observed in non-tumor cells. G Schematic representation of rearrangement in case 3 using BrassVis [69].

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