Fig. 1: Suppression of MNX1 expression mediated by 5-aza-2’-deoxycitidine (DAC).

A Cell viability after treatment with the 50 most effective epigenetic compounds (refer to Supplementary Table 1). Green bars refer to compounds selected for further analysis, number 17 refers to DAC. B MNX1 mRNA expression was measured by qRT-PCR after treatment with ten selected compounds. The DMSO treatment was used for normalization. Quantification was performed using 2-Δ(Ct) method with GAPDH Ct-values, and the relative expression values from the compound treated and DMSO treated samples were divided by the average relative DMSO expression. These calculations were repeated for each compound. Expression after DAC treatment was reduced to 0.55-fold (Two-tailed T test. *p < 0.05) C Volcano plot showing transcriptional changes after DAC treatment. Red dots indicate significantly up- or downregulated transcripts. Genes with a false discovery rate (FDR)-adjusted p value (based on DESeq2) of less than 0.05 and an absolute (log2(fold change)) of greater than 1 were classified as significant. MNX1 and cancer testis antigens are highlighted. D Representative Western blot of MNX1 protein levels from GDM-1 cells extracted 120 h after DAC treatment. E Quantification of Western Blot in D. The p values come from a one-tailed t test.