Fig. 2: Activated miR-200a-3p suppresses MNX1 in GDM-1 and MNX1 expressing PDX samples.

A Volcano plot of miRNA-seq expression data from GDM-1 cells treated with DAC. Genes with a false discovery rate (FDR)-adjusted p-value (based on DESeq2) of less than 0.05 and an absolute (log2(fold change)) of greater than 1 were classified as significant. B Representative Western blot (left) and quantification (right) showing MNX1 protein expression in GDM-1 cells upon treatment with miRNA-200a-3p mimic (miRNA-200a-3p) or scrambled control (miR-NC). Each of the three biological replicates was normalized to its specific control experiment. C Luciferase signal intensity in HEK293 cells with mimics for miR-200a-3p and negative control (used for normalization for each of the replicated separately), NC. D Quantification of MNX1 transcription in PDX491 and PDX661 after DAC treatment through qRT-PCR. The mean of the DMSO replicates was used for normalizing DAC-treated PDX samples. E Representative Western blot (left) and quantification (right) showing reduced MNX1 protein levels in DAC-treated PDX491 and PDX661. F Log2 of miR-200a-3p expression levels in DAC-treated PDX491 and PDX661. B–F The p values were obtained from one-tailed t-tests.