Fig. 1: Description of the 3D vasculature-like model for leukemic PDXs. | Leukemia

Fig. 1: Description of the 3D vasculature-like model for leukemic PDXs.

From: A three-dimensional ex vivo model recapitulates in vivo features and drug resistance phenotypes in childhood acute lymphoblastic leukemia

Fig. 1

A Experimental set-up. The 3DProSeed® Hydrogel Well Plate was used for the establishment of the ex vivo model. Primary MSCs are cultured for 24 h, followed by the addition of HUVECs, MSCs and ALL PDXs. B Representative confocal fluorescence images of the co-cultures in maximum intensity projections using confocal z-stacks (z-step: 10 μm) showing MSCs (blue), HUVECs (green), and ALL cells (red). C, D A vasculature-like network is detected in a similar manner in all 11 conditions tested (MSC-HUVEC controls, 10 PDXs co-cultures with MSC and HUVECS). Depicted are the (C) mean volume and (D) mean surface of the HUVECs (“vasculature”) in triplicates. Controls were quantified in triplicates in three separate experiments (statistical analysis: unpaired t test, significant p value < 0.05).

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