Fig. 3: MSC multi-lineage differentiation in 3D resembles in vivo and human data. | Leukemia

Fig. 3: MSC multi-lineage differentiation in 3D resembles in vivo and human data.

From: A three-dimensional ex vivo model recapitulates in vivo features and drug resistance phenotypes in childhood acute lymphoblastic leukemia

Fig. 3

A UMAP projections showing MSC population overlap based on the culturing condition; left: two controls, i.e. MSC and MSC ( + HUVEC), right: MSC ( + HUVEC + ALL), PDX1-PDX6 indicating the six different ALL PDXs). B Feature plots depicting the expression of CXCL12 (top) and PDGFRA (bottom) across the MSC populations. C Identification of multi-lineage MSC subpopulations based on established lineage-specific gene signatures, projected in UMAP, upon coculturing in 3D. D Proportional distribution of MSC subtypes across conditions, with the increased population percentages in white. Arrows indicating increase or decrease upon co-culture. E GSEA dot plot showing the top 10 enriched pathways in MSCs from the MSC( + HUVEC) and MSC( + HUVEC + ALL) to the MSC control comparison.

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