Fig. 7: Drug screenings in the 3D co-culture reveal increased resistance. | Leukemia

Fig. 7: Drug screenings in the 3D co-culture reveal increased resistance.

From: A three-dimensional ex vivo model recapitulates in vivo features and drug resistance phenotypes in childhood acute lymphoblastic leukemia

Fig. 7

A Gating strategy for flow cytometry. Viable ALL cells were gaited for lymphocytes based on their size, followed by doublet exclusion, cellTrace positivity and PI negativity. B Experimental design. Drug responses in the 3D co-culture system were compared to those in a standardized drug response profiling protocol, where immortalized hTERT-MSCs-ALL co-cultures are treated for 72 h (n = 3). C Bar plots showing the normalized response values (integrated normalized cell counts over two concentrations) for all the 3D and 2D conditions (n = 3, statistical analysis: ratio paired t test, p value < 0.05). D Percentile ranking indicating the response patterns across the conditions, color-coded based on the response in 3D. E–I Dot plots showing the normalized viability of the leukemic cells upon treatment with different compounds. Depicted is the response to 100 and 10,000 nM in the 2D and 3D systems respectively. From left to right : (E) idasanutlin, (F) venetoclax, (G) doxorubicin, (H) vincristine, and (I) dexamethasone.

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