Fig. 5: Deconvolution of RNA and methylome profiles. | Leukemia

Fig. 5: Deconvolution of RNA and methylome profiles.

From: Divergent molecular pathways drive monomorphic epitheliotropic and enteropathy-associated intestinal T-cell lymphoma

Fig. 5

A Barplots showing the means of cell-type fractions observed by deconvolution analysis in 48 MEITLs and 27 EATLs based on gene expression (upper part) and in 39 MEITLs and 26 EATLs based on methylation profiles (lower part). Cell type composition was calculated for each sample based on methylation values using the FlowSorted.Blood.EPIC R package. B Heatmaps representations of the differences in proportions of immune cell subtypes between EATL and MEITL for deconvolution analysis of RNA sequencing (left) and global DNA methylation (right) data expressed in log2(mean (EATL)/mean (MEITL)), *, p < 0.05; **, p < 0.01 and ***, p < 0.001. C Immunostainings for macrophage markers (CD68, CD163) and PD-L1 in one MEITL (left) and one EATL (right). D Boxplots showing the comparisons of CD68, CD163 and PD-L1 percentages of positive cells between EATL (n = 21) and MEITL (n = 54) samples. ***, p < 0.001 and ****, p < 0.0001. Differences between groups were assessed by two-way ANOVA with Sidak’s multiple comparisons test.

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