Fig. 3: The use of OPT attenuation maps to correct artifacts in fluorescent SPIM data—isosurfaces.
From: Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM
The directions of illumination (ill) and detection (det) are indicated; the sample is the E12.5 embryonic mouse head from Fig. 1. a Overview of the region of the sample that experiences significant attenuation from eye pigmentation (cyan). The volumes strongly affected by attenuation are indicated by the gray quasi-cylindrical translucent structures (illumination attenuation extends roughly to the right from the eyes; detection attenuation is approximately into the plane of the image). Magenta: Unattenuated fluorescence; green: regions of fluorescence that experienced significant attenuation and were corrected. b, c Visualizations of the sample looking into the detection direction behind one eye (cyan): b before attenuation correction and c after correction. Yellow arrowheads indicate missing structures in the uncorrected data that are restored after correction (orange arrowheads)
