Fig. 4: The use of OPT attenuation maps to correct artifacts in fluorescent SPIM data—optical sections.
From: Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM
The directions of illumination (ill) and detection (det) are indicated; the sample is the E12.5 embryonic mouse head from Figs. 1 and 3. Optical sections from the fluorescent SPIM data set of approximately the same region shown in Fig. 1e. Images are shown before (a) and after (b) attenuation correction. The yellow bracket indicates the region for which the excitation is strongly affected by attenuation in (a) but is well corrected in (b) (orange bracket). c The same region imaged after rotation of the sample by 90°. The fluorescence to the right of the eye is unattenuated and serves as a control to illustrate what the corrected data in b should look like. Scale bar: 500 µm
