Fig. 2: Ca2+-activated force and SHG multiphoton imaging simultaneously performed in single wt and mdx EDL muscle fibers. | Light: Science & Applications

Fig. 2: Ca2+-activated force and SHG multiphoton imaging simultaneously performed in single wt and mdx EDL muscle fibers.

From: Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach

Fig. 2

Representative example recordings of force (top) and myosin SHG signals (bottom) from a single EDL fiber from a wt mouse (left) and an mdx mouse (right) during successive solution exchange for increasing Ca2+ concentrations (decreasing pCa). Brief positive force spikes represent the time points of manual solution exchange performed several times per pCa step. Scale bar: 20 µm. CAS: cosine angle sum (a.u.). VD: vernier density (#/100 µm2)

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