Fig. 1: Schematic representation of the hybrid microscopy system, which consists of a subsystem for dual-wavelength optoacoustic microscopy at 488 nm and 808 nm co-aligned with a subsystem for multiphoton microscopy at 1043 nm.
From: Optoacoustic microscopy at multiple discrete frequencies
a AMP amplifier, CCD bright-field camera, DAQ data acquisition card, DM dichroic mirror, GC galvanometric mirror controller, IQD IQ demodulator, LO1 local oscillator 1, LO2 local oscillator 2, NDF neutral density filters, OA optoacoustic, OF optical filter, PC personal computer, PH pinhole, PMT photomultiplier tube, SHG second-harmonic generation, THG third-harmonic generation, TPEF two-photon excitation fluorescence, xyz motorized stages. b The spectrum of the excitation and detection wavelengths in hybrid FDOM/multiphoton (MP) imaging. c Schematic comparison between time-domain (TD) optoacoustic microscopy, which uses short pulses of light, and frequency-domain (FD) optoacoustic microscopy, which is based on a modulated laser intensity at multiple discrete frequencies (the figure is only for representation purposes; it is neither scalable nor quantitative)
