Fig. 1: Resolving the NPC.
From: Sharpening emitter localization in front of a tuned mirror
a Optimized metal-dielectric substrate designed to exhibit the strongest enhancement field for emission (solid line) and excitation (dashed line) of the red emitting dye A647 at the axial position of the NPC ring structure labeling the pore anchoring protein gp210. b Simulated conventional (localization precision: 20 nm, left) and mirror-enhanced dSTORM (12 nm, right) images; respective experimental image of a single NPC ring and overview images. c Fourier ring correlation (FRC) resolution estimation of sunny-side-down (gray solid line, see Supplementary Fig. S1), TIRF- (gray dashed line) and mirror-enhanced dSTORM (blue line) images of b. d–h Statistical analysis. d Principle of TRABI22. Histograms of e intensity distribution, f standard deviation, g background variance, and h resulting localization uncertainty of the localization events for coated coverslips (blue) with half (filled bars) and the same (open bars) excitation power as in the experiments on uncoated coverslips (gray) in the sunny-side-down (filled bars) and TIRF configurations. The inset in h highlights the increased number of events with a localization uncertainty below 10 nm for coated (blue) versus uncoated coverslips in the sunny-side-down (gray filled bars) and TIRF (gray open bars) configurations. Scale bars: 50 nm (single rings) and 1 µm (overview)
