Fig. 2: PV-1 efficiently delivers diverse cell-impermeable organic fluorescent probes into live cells. | Light: Science & Applications

Fig. 2: PV-1 efficiently delivers diverse cell-impermeable organic fluorescent probes into live cells.

From: Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging

Fig. 2

a Confocal microscopy images of live U-2 OS cells after a 1-h coincubation with PV-1 (4 μM) and the indicated probes for microtubules with different colors (5 μM). Scale bars: 10 μm. b Confocal microscopy images of live U-2 OS cells after a 1-h coincubation with PV-1 (4 μM) and Hoechst-Alexa 488 or Hoechst-Alexa 647 (3 μM). Scale bars: 20 μm. c Confocal microscopy images of live U-2 OS cells after a 1-h coincubation with PV-1 (4 μM) and Morph-Atto 488 or Morph-Alexa 647 (5 μM). Scale bars: 5 μm. The labeled organelles are indicated in the bottom left of the first images. d Confocal microscopy images of live U-2 OS cells expressing SNAP-Sec61β (top row, four first images), CLIP-Sec61β (top row, last image), SNAP-Actin, SNAP-Cox8a, SNAP-H2B, CLIP-Lifeact or CLIP-Actin after a 1-h coincubation with PV-1 (4 μM) and the indicated SNAP or CLIP probes (5 μM). Scale bars: 5 μm

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