Table 2 NA values and measured optical resolutions for compound assemblies using DOs of different models (Fig. 1g) and the ‘standard version’ MO (Table 1).
From: MATRIEX imaging: multiarea two-photon real-time in vivo explorer
DO magnification and model | ×5 Olympus MPlan N | ×4 Olympus MPlan N | ×2.5 Olympus MPlan FL N | ×2 Mitutoyo MPlan Apo |
|---|---|---|---|---|
Diameter of the target zone | 4.8 mm | 6 mm | 9.6 mm | 12 mm |
NADO given by the manufacturer | 0.1 | 0.1 | 0.08 | 0.055 |
NAeff of the compound assembly | 0.48 | 0.48 | 0.48 | 0.43 |
On-axis lateral resolution | 1.0 ± 0.1 μm | 1.1 ± 0.2 μm | 1.4 ± 0.1 μm | 1.5 ± 0.1 μm |
On-axis axial resolution | 35 ± 1 μm | 37 ± 3 μm | 42 ± 2 μm | 51 ± 3 μm |
Distance between MO and DO axes for off-axis measurements | 2 mm | 2 mm | 3 mm | 4 mm |
Off-axis lateral resolution | 1.3 ± 0.1 μm | 1.4 ± 0.3 μm | 1.6 ± 0.2 μm | 1.8 ± 0.2 μm |
Off-axis axial resolution | 46 ± 2 μm | 45 ± 2 μm | 48 ± 1 μm | 54 ± 3 μm |
