Fig. 4: Comparison of in vivo MSOM images of three breast cancer tumour types and MSOM-based in vivo quantitation of sO2 and HbT heterogeneity throughout entire tumours.
a The upper row shows representative maximum intensity projections of total haemoglobin concentration (HbT) in the xz plane of entire tumours in vivo. b, c Maximum intensity projections of the distribution of (b) HbT and (c) sO2 in the xy plane of the tumours, as defined by the dashed lines in panel (a). d–k Various heterogeneity metrics were measured in two biological replicates of the three types of breast cancer tumours. Details of how metrics were calculated can be found in the Methods section, together with an explanation of how tumours were segmented into centre and rim regions. MDA refers to MDA-MB-231. Some images have been rotated to highlight correlations with other images. All maximum intensity projections in this figure were calculated over sections with a thickness of 400 μm. Scale bars, 1 mm
