Fig. 2: Examples of various fields of view that have been virtually stained using the presented multistain network.

Co-registered histochemically stained fields of view of the same samples are also shown to the right, and the unstained autofluorescence images are shown to the left to permit direct comparison. a, b Tissue stained with H&E, c, d tissue stained with Masson’s trichrome, and e, f tissue stained with Jones’ silver. Contrast-enhanced unstained tissue images are provided for visual guidance; unprocessed raw versions of these images were used as the input to the neural network