Fig. 1: AO-TPEFM enables non-invasive subcellular imaging of the mouse retina. | Light: Science & Applications

Fig. 1: AO-TPEFM enables non-invasive subcellular imaging of the mouse retina.

From: Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Fig. 1

a Schematics of in vivo AO-TPEFM of the mouse retina using direct wavefront sensing from a two-photon fluorescence guide star. b Representative spot diagram collected by the SHWS. c Representative aberration wavefront of the mouse eye (unit: μm). d, e The lateral (d) and axial (e) resolution of the AO-TPEFM system after full AO correction. The mean values of the measured FWHM from six locations are shown in the figures. f Depth-resolved imaging of different retinal layers with system (top) or full (bottom) AO correction. g Mosaic TPEF images of RGCs (green) and blood vessels (red) with system (left) and full (right) AO correction. h Signal profiles along the dashed lines in (g) for a comparison of the fluorescence intensity of RGCs with system (red line) and full (black line) AO correction

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