Fig. 2: In vivo functional calcium imaging of RGCs. | Light: Science & Applications

Fig. 2: In vivo functional calcium imaging of RGCs.

From: Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Fig. 2

a Schematics for the calcium imaging experiment. b Two-photon imaging of RGCs labelled with AAV9-hsyn-GCaMP6s. Dendrites are finely resolved after full AO correction. c Calcium transients of RGC dendrites marked in (b). The vertical dashed lines indicate the timing of blue light flash stimulation. d Two-photon fluorescence images of the GCaMP-labelled RGCs with the system and full AO correction. e Calcium response of cells of interest (marked in (d)) with system and full correction. The left plane indicates the change in fluorescence intensity relative to the resting fluorescence intensity (∆f/f), and the right plane shows the averaged calcium response and the decay half-life. f Calcium imaging with full AO correction reveals different types of RGCs

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