Fig. 3: Study microglial dynamics and function in the mouse retina. | Light: Science & Applications

Fig. 3: Study microglial dynamics and function in the mouse retina.

From: Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Fig. 3

a Mosaic TPEF projection images of microglia (green) and blood vessels (red) with system and full AO correction in the retina of Cx3Cr1-GFP transgenic mice. b Enlarged images of microglia and vascular structures in different retinal layers corresponding to the white dashed box in (a). c Signal intensity profiles of blood vessels and microglia along the dashed lines in (a). d Time-lapse images of retinal microglia and blood vessels before NMDA administration and four and eight hours after NMDA administration. The lower right corner shows the change in the ramification index of retinal microglia in response to NMDA administration. Each time point involves more than 20 microglia from three mice. e Time-lapse imaging showing the dynamics of the microglial process four hours after NMDA administration. The arrowheads mark the mobility of a microglial process. f Representative two-photon images showing the recruitment of round leukocytes eight hours after NMDA treatment. Arrowheads and arrows: two GFP+ leukocytes migrating along a retinal blood vessel; asterisks: two microglia attached on the endothelia of the blood vessel

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