Table 1 Quantitative measurements in 3D reconstructed tomograms of plant cells nuclei.
From: Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography
MO = 25× λ = 532 nm | MO = 20× λ = 632.8 nm | Mean ± std (*) | |||||
|---|---|---|---|---|---|---|---|
Cell 1 | Cell 2 | Cell 3 | Cell 4 | Cell 5 | Cell 6 | ||
Nuclear volume [μm3] | 1377.42 | 1085.82 | 2664.99 | 1954.09 | 3275.01 | 3102.74 | 2066.85 ± 844.14 |
Nuclear equivalent radius [μm] | 6.90 | 6.38 | 8.6 | 7.76 | 9.21 | 9.05 | 7.75 ± 1.14 |
Nuclear 1st principal axis [μm] | 19.61 | 19.22 | 25.22 | 20.63 | 24.73 | 23.74 | 21.58 ± 3.87 |
Nuclear 2nd principal axis [μm] | 13.89 | 14.97 | 14.35 | 13.79 | 16.00 | 15.50 | 14.50 ± 1.98 |
Nuclear 3rd principal axis [μm] | 6.98 | 5.26 | 10.14 | 9.51 | 11.41 | 11.58 | 8.84 ± 2.22 |
Nuclear average RI | 1.39 | 1.39 | 1.38 | 1.38 | 1.38 | 1.38 | 1.38 ± 0.01 |
Nuclear standard deviation RI | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 ± 0.00 |
Nuclear minimum RI | 1.35 | 1.35 | 1.36 | 1.35 | 1.35 | 1.35 | 1.35 ± 0.01 |
Nuclear maximum RI | 1.43 | 1.42 | 1.42 | 1.42 | 1.41 | 1.40 | 1.42 ± 0.01 |
Nuclear dry mass [pg] | 370.51 | 295.25 | 599.47 | 482.00 | 722.34 | 660.85 | 498.90 ± 180.80 |
Nucleolar volume [μm3] | 50.25 | 40.12 | 82.69 | 55.63 | 101.97 | 106.88 | 69.62 ± 27.24 |
Nucleolar equivalent radius [μm] | 2.29 | 2.12 | 2.70 | 2.37 | 2.90 | 2.94 | 2.51 ± 0.35 |
Nucleolar 1st principal axis [μm] | 4.95 | 4.53 | 5.32 | 4.93 | 5.67 | 6.18 | 5.20 ± 0.67 |
Nucleolar 2nd principal axis [μm] | 3.96 | 3.77 | 5.14 | 3.99 | 5.13 | 4.93 | 4.40 ± 0.62 |
Nucleolar 3rd principal axis [μm] | 3.56 | 3.25 | 4.21 | 3.90 | 4.85 | 4.82 | 4.01 ± 0.69 |
Nucleolar average RI | 1.41 | 1.41 | 1.40 | 1.39 | 1.39 | 1.38 | 1.40 ± 0.01 |
Nucleolar standard deviation RI | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 ± 0.00 |
Nucleolar minimum RI | 1.37 | 1.38 | 1.38 | 1.37 | 1.37 | 1.35 | 1.37 ± 0.01 |
Nucleolar maximum RI | 1.43 | 1.42 | 1.42 | 1.42 | 1.41 | 1.40 | 1.42 ± 0.01 |
Nucleolar dry mass [pg] | 17.95 | 14.58 | 26.77 | 16.27 | 31.07 | 26.03 | 21.97 ± 8.12 |
Nucleolar-nuclear volume ratio [%] | 3.65 | 3.70 | 3.10 | 2.85 | 3.11 | 3.44 | 3.41 ± 0.31 |
