Fig. 1: Dependence of fluorophore’s lifetime on chromatin replication timing.
From: Fluorescence lifetime imaging for studying DNA compaction and gene activities
a Representative fluorescence lifetime images of early, mid, and late S-phase DNA replication sites labeled with AlexaFluor 546. Corresponding lifetime histograms are demonstrated in Fig. S3. b Color-coded image of the lifetime distribution throughout cell nucleus. c Schematics of the FLIM approach to measure compaction of chromatin replicating at different S-phase windows. d Averaged fluorescence lifetime of AlexaFluor 546 used to label DNA replication sites in early, mid, and late S-phase. The error bars correspond to standard deviations. See Fig. S4 for additional examples
