Fig. 3: Schematic illustration of the FLIM-FRET assay to study genomic DNA compaction.
From: Fluorescence lifetime imaging for studying DNA compaction and gene activities
a Basic FLIM-FRET mechanism. b Direct labeling of the genomic DNA by incorporation of fluorophore-tethered nucleotides. c Relation between chromatin condensation levels and FRET efficiency. Scheme of simultaneous (d) and sequential labeling of the chromatin domains (e) during the S-phase of the cell cycle
