Fig. 3: Comparison of halftone-SFDI with continuous-tone SFDI on in vivo human tissue measurements.

a Illustration of measurements with the continuous-tone SFDI and halftone-SFDI methods. b Planar image of in vivo human hand tissue at 650 nm. c The tissue optical properties were measured with the two methods from 650 nm to 850 nm with 50 nm increments. d The average and standard deviation of the extracted optical properties are compared for the entire maps extracted by the two methods. e Functional chromophore maps were calculated from absorption spectra based on Beer’s law. f The differences in extracted hemoglobin concentrations were visualized and quantitatively compared