Fig. 5: High-speed quantitative monitoring of highly dynamic flow field. | Light: Science & Applications

Fig. 5: High-speed quantitative monitoring of highly dynamic flow field.

From: Halftone spatial frequency domain imaging enables kilohertz high-speed label-free non-contact quantitative mapping of optical properties for strongly turbid media

Fig. 5

a. A 8 × 6 × 3.5 cm silicone phantom was fabricated with a 6 × 4 × 2.5 cm well as container. The well was initially filled with liquid phantom (60 ml 1% intralipid) before monitoring. b. The halftone-SFDI was used to monitor the center of the liquid phantom with a 1 × 0.7 cm field of view. The monitoring was conducted at 1000 Hz with 470 nm and 625 nm wavelengths. A dynamic flow with absorption and scattering contrasts was induced by injection of a solution made with nigrosin and 20% intralipid. c The extracted absorption and reduced scattering maps, as well as chromophore maps at three representative time points are demonstrated for the quantitative monitoring of the flow field. A complete video showing the process is included as supplementary file. (The video was compressed and converted from original frames to meet the upload requirement)

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