Fig. 5: Aberration-free volumetric high-speed imaging of a mouse brain.
From: High-throughput volumetric adaptive optical imaging using compressed time-reversal matrix
a Imaging configuration. The mouse brain tissue was continuously scanned along the z-axis at a constant speed while dynamically varying speckle patterns illuminated the specimen. b Reconstructed 3D volumetric image of brain tissue with transverse field of view of 128 × 128 µm and depth range of 125 µm, composed of 568 × 568 × 125 voxels. The two white dashed curves indicate wall boundaries of a blood vessel with a diameter of ~30 µm, located close to the brain surface. c Left-hand and middle columns show MIP images with and without aberration correction at various depths, respectively. MIP range: 6 µm for z = 15, 65, and 85 µm, and 20 µm for z = 115 um. The right-hand column shows reconstructed output pupil phase maps for 11 × 11 subregions. The white dashed curves in the uncorrected image at z = 15 µm indicate wall boundaries of a blood vessel near the brain surface. The radius of each circle corresponds to a numerical aperture of NA = 0.94. The images at each depth were normalized by the maximum intensity in the corrected image. Scale bar: 20 µm. d Line profiles along the vertical white dotted lines in the images at z = 115 µm in (b)
