Fig. 4: Receptive field mapping in auditory neurons with coverage of low sound levels.
From: A silent two-photon imaging system for studying in vivo auditory neuronal functions
a Calcium traces of three exemplar neurons. Trials vary in pure-tone stimulus frequency and level. The experiment covers sound levels down to 5 dB SPL. Solid black lines: mean responses (n = 10 repetitions); light gray lines: responses of individual trials. b The FRA (frequency responsive area) summarized from the calcium responses in (a). These exemplar neurons show FRAs with the characteristic types of V-shape, I-shape, and O-shape. c More exemplar FRAs of these characteristic types from nine additional neurons. Among these exemplar FRAs. Clear responses at the sound level of 20 dB SPL are common. d The topography of neurons in the FOV labeled with their best frequencies (BFs). e The histogram of BF distribution. f The topography of neurons in the FOV labeled with their best levels (BLs). Scale bar: 100 μm. g The histogram of BL distribution
