Fig. 3: Experimental verification of learned deconvolution in beads. | Light: Science & Applications

Fig. 3: Experimental verification of learned deconvolution in beads.

From: Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Fig. 3

a–d show the illumination beam and the corresponding acquired LSM image (LR), which is deconvolved using Richardson–Lucy (RL) and deep-learning (DL) methods. a Gaussian, b Airy (α = 0.5), c Airy (α = 1) and d Airy (α = 2) beam. Insets and profiles correspond to the bead marked by a red square. Red dashed lines mark the Rayleigh range of each beam. Scale bars are 50 μm for images and 5 μm for plots. e Autocorrelation function (ACF) and f modulation transfer function (MTF) with respect to depth of the Gaussian and Airy (α = 1) LSM images

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