Fig. 7: Learned deconvolution in a multiphoton LSM with a Bessel beam. | Light: Science & Applications

Fig. 7: Learned deconvolution in a multiphoton LSM with a Bessel beam.

From: Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Fig. 7

a Fluorescent beads encoded by a Bessel PSF (LR) deconvolved using deep learning (DL). Zoomed-in regions correspond to areas marked by the red rectangle. Line plots are representative of the axial intensity profile, showing deconvolution of side lobes. b–f Zebrafish embryo sections: b cross-sections of the iris; c enface sections of the pupil; d cross-sections of the body; e enface maximum intensity projections (20 μm) of the body. Line insets correspond to intensity profiles along the regions marked by red triangles. In (c), red triangles illustrate out-of-plane defocus. f Maximum intensity projections of the entire sample in the cross-section and enface planes. Red rectangles mark locations corresponding to (b–e). Scale bars are 50 μm in (a–e) and 200 μm in (f)

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