Fig. 1: RASTMIN setup illustration and the main modulation patterns in some selected sequentially structured illumination(SSI)-based techniques. | Light: Science & Applications

Fig. 1: RASTMIN setup illustration and the main modulation patterns in some selected sequentially structured illumination(SSI)-based techniques.

From: Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

Fig. 1

a RASTMIN setup consists of excitation with donut modulation, xy-scan, drift correction and time-related single photon detection modules which has high compatibility with scanning-based fluorescent microscope, if starting from the experience on STED construction. b Structured excitation in 2D-MINFLUX13, pulsed-MINFLUX15, 3D-MINFLUX14, MINSTED21, SIMFLUX20, ROSE19, ModLoc22, and RASTMIN16, respectively. *Regular focus is not displayed. **Only crucial coherent modulation is illustrated; the delicate control and difference are not displayed.

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