Table 1 List of buffers with recipes

PCA/Trolox

2 mM Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)

12 mM PCA (protocatechuic acid)

12.5 mM MgCl₂·6H₂O

40 mM Tris base

20 mM acetic acid

1 mM EDTA-Na₂·2H₂O

PAINT PCA/Trolox

2 mM Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)

2.4 mM PCA (protocatechuic acid)

6 mM MgCl₂·6H₂O

40 mM Tris base

20 mM acetic acid

1 mM EDTA-Na₂·2H₂O

50 × PCD

2.8 mM PCD (protocatechuate 3,4-dioxygenase from pseudomonas sp.)

50% glycerol

50 mM KCl

100 mM Tris HCl

1 mM EDTA-Na2·2H₂O

FOB

12.5 mM MgCl₂·6H₂O

20 mM Tris base

20 mM acetic acid

1 mM EDTA-Na₂·2 H₂O