Fig. 3: Infrared laser damage evaluation.
From: Soft monolithic infrared neural interface for simultaneous neurostimulation and electrophysiology
a Microscope image of a brain slice used for IHC, stained with Iba-1 (yellow) and DAPI (cyan), with the ROIs used for analysis indicated as dashed lines. b Average fluorescence intensities for Iba-1 and CCR2 in the large ROI (N slices > 5). c Magnification of 2a showing the small ROI only; the dashed lines indicate the position of the fibre during stimulation. d Colorized depiction of a small ROI’s subdivision into insertion and stimulation regions (Iba-1 channel only). e Average fluorescence intensities for Iba-1 and CCR2 in the small ROI (N slices > 5). f Average fluorescence intensities for Iba-1 in the insertion and stimulation regions of the small ROI (N slices > 5). g, h Magnified images showing microglia and monocytes in the implantation region, respectively
