Fig. 2: DEEP2 validation results on Synthetic Fluorescent Beads at 4 scattering lengths (SLS) below the surface.
From: DEEP-squared: deep learning powered De-scattering with Excitation Patterning
Synthetic beads-objects of the size of 1 and 4 μm, with the intensity of the 1 μm beads 5× higher than the 4 μm beads, were simulated. Their corresponding simulated DEEP-TFM image stacks were generated using the forward model. A subset of the data was used to train the DEEP2 inverse model, and the remaining unseen data were used to validate the model performance. a, d Two representative simulated DEEP-TFM image stacks (averaged over the 32 patterns). b, e Corresponding synthetic ground truths for the (a) and (d) instances. c, f DEEP2 reconstructions corresponding to (a) and (d) instances. The intensity along the lines G, H, and I shown in green, yellow and white on (a) is visualized in (g–i). Similarly, the intensity along the lines J, K, and L are shown in (j–l) plots
