Fig. 2: The maximum occupation of sub-ROI testing, and the framerate demonstration with QXGA and SXGA series of SLM under 2 Angles 3 Phases or 3 Angles 3 Phases SIM excitation. | Light: Science & Applications

Fig. 2: The maximum occupation of sub-ROI testing, and the framerate demonstration with QXGA and SXGA series of SLM under 2 Angles 3 Phases or 3 Angles 3 Phases SIM excitation.

From: Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)

Fig. 2

a QXGA for maximum occupation test, the max sub-ROIs are 192 pixels (H)×676 pixels (W) in 0.2 ms 1-bit balance pattern exposure when the frame opens 400 pixels (H) × 2048 pixels (W), and b 236 pixels (H)×676 pixels (W) in 0.4 ms 1-bit balance mode when the frame opens 496 pixels (H)×2048 pixels (W). The maximum in SXGA (c) can be around 234 pixels (H)×676 pixels (W) when the frame is 484 pixels (H)×2048 pixels (W) in 0.2 ms, and d 296 pixels (H)×676 pixels (W) in 0.4 ms displaying when the frame opens 608 pixels (H)×2048 pixels (W). In the framerate demonstration, the correct angles are illustrated by FFT hot map containing counterpart of ±1 order surefire directions circled with white, which are consistent with the direction of red arrows on top-right part of each sub-ROI. The fringe phases are corrected by checking successful reconstruction. c Sub-ROIs’ size in SXGA group is only for pattern displaying demonstration. c the sub-ROIs are 192 pixels (H)×400 pixels (W) in 0.2 ms 1-bit balance displaying mode when the frame opens 484 pixels (H)×2048 pixels (W), and d 260 pixels (H)×540 pixels (W) in 0.4 ms 1-bit balance mode when the frame opens 608 pixels (H)×2048 pixels (W). Using the QXGA, 0.2 ms and 0.4 ms PAR-SIM framerate are 256 Hz and 204 Hz, respectively, when under 2 Angles 3 Phases SIM mode; and corresponding to 171 Hz and 136 Hz under 3 Angles 3 Phases SIM. Meanwhile using SXGA, 0.2 ms and 0.4 ms PAR-SIM framerate are 209 Hz and 167 Hz, respectively, when under 2 Angles 3 Phases mode; and with respect to 140 Hz and 112 Hz under 3 Angles 3 Phases mode. All the framerates from the whole frame above almost match their respective theoretical opened row numbers on the sensor because of the flexible synchronization margin mentioned above. Scale bars: 2 μm

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