Fig. 3
rPMT-based single-shot microscopic imaging for biological microscope slides and dynamic live samples at a meter-scale working distance. a The retrieved amplitude (i.e., the normalized transmittance) of a cyclop at an object-to-screen distance of ZOS = 913.0 mm. The detailed region marked by the green dotted circle reveals clear identification of each caudal rami of the cyclop. b The retrieved phase contrast image of the cyclop, providing insight into high-transmittance regions in (a), such as the region indicated by the red dotted ellipse. The phase information can aid in the identification of these regions. c Transverse section of a young root tip of broad bean at ZOS = 1084.3 mm. d Detailed region cropped from (c). e The transverse section of a female roundworm with a FOV of 1.9 mm diameter. The detailed region marked by the red dotted circle clearly identifies the longitudinal muscle cells. f Two sections of a single tenia proglottid measuring 3.9 mm in length, with a FOV of 2.9 mm diameter. The detailed region marked by the green dotted circle clearly identifies the vacuoles of the cytoplasmic region. g The same tenia proglottid illustrated in (f), situated within a single FOV of 4.7 mm diameter at ZOS = 1072.2 mm. h The retrieved image of three live cyclops with a 3.3 mm-diameter FOV at ZOS = 1080.5 mm. The region indicated by the green dotted circle distinctly highlights the antennal spines on the first antenna, whereas the yellow dotted circle reveals clear caudal ramus and the egg sacs. i One frame from a reconstructed video featuring a live Moina mongolica (indicated by the blue solid circle) alongside a Simocephalus vetulus, which SPS image sequence was captured at a frame rate of 40 fps (refer to Supplementary Video 1 for the full reconstructed video). The region indicated by the red dotted circle distinctly highlights the rod-shaped antennal spines on the antenna of the Simocephalus vetulus. j Four additional frame of the video, captured at 25.0 ms, 149.8 ms, 2072.5 ms, and 2222.3 ms, subsequent to the frame illustrated (i). These figures exclusively display the ROIs, indicated by the blue solid circles in i, which illustrate the postural changes of Moina mongolica. k One frame from a reconstructed video showcasing two live cyclops alongside two Caenorhabditis elegans, which SPS image sequence was captured at 74 fps (refer to Supplementary Video 1 for the full reconstructed video). l Four additional frame of the video, captured at 13.5 ms, 54.0 ms, 81.0 ms, 108.0 ms following the frame shown in (k). These figures focus exclusively on the ROIs, denoted by the pink solid circles in (k), which clearly illustrate the jumping process of the small cyclop on the egg sacs of the larger cyclop with a time sampling interval of 13.5 ms. (refer to Supplementary Video 1 for details)
