Fig. 2: Spatially multiplexed single-molecule FLIM of labeled membrane proteins.
From: Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera
a Image of Cy3B-labeled aerolysin proteins in a supported lipid bilayer, showing detected molecules (yellow square) and picked molecules (red square) based on their suitability for lifetime assignment. b Structure of the pore-forming aerolysin protein and position of the label. c Lifetime distributions obtained from 5 fields of view for LD555 (n = 1643), Cy3B (n = 2380) and AF488 (n = 301) dyes, respectively. For each distribution the total acquisition time is less than 1 min. The gate delay is 2.5 ns for LD555 and Cy3B and 3 ns for AF488
