Fig. 3: Optimization of the acquisition scheme.
From: Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera
a Experimentally varying parameters using fluorescent bead imaging with controlled gate delay T and controlled background resulting in different SBR values. b Experimental F-values are compared with the theoretically expected formula \(F(u,b)=\sqrt{2(1+b)(1+b+{e}^{u}+b{e}^{2u})}/u\) where b = 1/SBR and u = T/τ is the normalized gate delay. c Optimal gate delay (top) and minimum F-value at this optimal delay value (bottom) as a function of the emitter’s SBR. Solid lines are numerically evaluated from deriving the minimum of the F-value formula, and markers indicate experiments
