Fig. 1: Schematic of ISM-FLUX.
From: Localization of single molecules with structured illumination and structured detection
a Sequential structured illumination with a toroidal beam in ISM-FLUX, b Structured detection corresponding to each of the sequential exposures. c Schematics of the experimental setup. Based on a standard confocal microscope, ISM-FLUX includes a vortex-phase plate (VPP) in the excitation path to generate a toroidal (doughnut-shaped) focus, and a SPAD array in the detection that is connected to a time-correlated single-photon counting (TCSPC) unit to perform spectroscopic measurements
