Fig. 4: Rapid two-dimensional characterisation of a mixture of bovine serum albumin and human lysozyme.
From: Rapid two-dimensional characterisation of proteins in solution
The quantitative nature of the eletrophoretic separation unit allowed the applied potential to be related to the electrophoretic mobility μel of each of the fractions. These data were used in combination with the extracted hydrodynamic radii (Rh) to estimate the effective charges (q) of the molecules in each of the fractions and to construct a two-dimensional q vs Rh map of the mixture over a few minute timescale by monitoring the device only at a single imaging frame
