Fig. 6: Separation and identification of CTCs from patient blood.
From: Manipulation of single cells via a Stereo Acoustic Streaming Tunnel (SteAST)
a Process of single CTC separation from patient blood. The microchannel consists of three inlets and one outlet. The direction of flow is shown by white arrows. After CTCs are trapped in SteAS, dye is injected to achieve immune-based identification. The identified cells were washed to improve purity. b Process of staining and washing step. The trapped cells were stained green and red, which means that the cells demonstrated good viability and were CTCs from the perspective of immunology. The scale bars in the images and detailed images are 100 μm and 50 µm, respectively.
