Fig. 5: Detection of drug-induced shifts in mitochondrial function. | Microsystems & Nanoengineering

Fig. 5: Detection of drug-induced shifts in mitochondrial function.

From: Measurement of oxygen consumption rates of human renal proximal tubule cells in an array of organ-on-chip devices to monitor drug-induced metabolic shifts

Fig. 5

a Representative images of hRPTECs stained for nuclei (blue) and actin (red) showed no significant changes in cell density following a 1.5-hour exposure to either 1.5 μM Antimycin A (Anti), 1.5 μM Oligomycin (Oligo), or 2 μM FCCP (scale bar: 500 μm). b Representative oxygen depletion curves (symbols) with simulated curve fits (black line) for single devices in the control, Anti, Oligo and FCCP groups. c Single-device OCR measurements acquired pre- and postdrug exposure at t = 61.4 minutes (dotted line). d OCR following drug exposure for each condition. Data are from 3–4 devices per condition. Error bars represent standard deviation, *p < 0.05, **p < 0.01 with respect to control (single-factor ANOVA with Tukey’s Post Hoc test)

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