Fig. 1: Schematic, workflow, images, and output results of piRT-IFC.
a Schematic of the crossing constriction channel-based impedance flow cytometry; b Raw impedance data acquisition with hardware and software facilities; c Cell detector used to detect cells and extract their impedance feature vectors; d Fast parallel physical fitting (FPPF) solver for obtaining intrinsic electrical properties Csm vs. σcyto; e Photograph of the piRT-IFC and microscopic image of the microfluidic chip embedded with a cross-shaped constriction channel; f Interface for users to control and watch output results; g Scatter plot of intrinsic electrical properties of single cells’ Csm vs. σcyto obtained in a five-minute cycle
