Fig. 2 | Microsystems & Nanoengineering

Fig. 2

From: Towards practical point-of-care quick, ubiquitous, integrated, cost-efficient molecular diagnostic Kit (QUICK) PCR for future pandemic response

Fig. 2

Overview of the PCR-based molecular diagnostic process. a Sample collection: Biological samples were collected from patients using nasopharyngeal swabs. b Sample preparation: Collected samples release NA through lysis, and RNA is then extracted and purify RNA using column-based methods. c RT-PCR amplification: Extracted RNA was converted to cDNA through reverse transcription and then subjected to thermal cycling for target gene amplification. d Detection: Amplified DNA is analyzed using gel electrophoresis, real-time fluorescence detection, or digital PCR

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