Table 1 Comparative analysis of different sample preparation methods
Sample preparation method | Driving power | External equipment | Sample type | Sample volume (µL) | Sample preparation time (min) | Extraction efficiency (%) | Ref. |
|---|---|---|---|---|---|---|---|
Microfluidic automation | Vacuum battery | X | Whole blood | 100 | 12 | 95 | |
Degas-driven flow/trench filter | X | Whole blood | 5 | < 10 | ~ 100 | ||
Gravity/Dielect rophoresis | X | Whole blood | 10 | 4 | 44 | ||
Paper-based extraction | Capillary action | X | Whole blood, serum, saliva, sputum | 30 | 2 | - | |
Capillary force/finger actuator | X | Whole blood | 3000 | 7 | - | ||
Centrifugal | Centrifugation | Centrifuge | Whole blood | 150 | 30 | - | |
Centrifugation | Centrifuge | - | 10 | 80 s | - | ||
Centrifugation | String | Whole blood | - | 90 s | - | ||
Ultrasonic lysis/Magnetic bead separation | Ultrasonic wave | Ultrasonic generator | Whole blood | 1 | 8 | ~60 | |
Magnetic extraction | Plate shaker | Nasopharyngeal swab | 30 | 30 | - | ||
Ultrasound | Ultrasonicator | Nasopharyngeal swab | 80 | 10 | 95.3 | ||
Magnetic extraction | Magnet | Whole blood | 4000 | - | 83.3 |
